Antibacterial Bioactivity of n-Hexane Extract from Mahogany (Swietenia humilis Zucc.) Seed and Its Fatty Acid Compound Identification

Penulis: AP Asmara, Hernawan,C Nuzlia, Roni Maryana
An investigation of antimicrobial bioactivity followed by GCMS analysis for the fatty acid composition has been conducted to the crude n-hexane extract of Swietenia humilis Zucc. seed. The goal of this research to determine the inhibition zone diameter for antimicrobial activity and the percentage of fatty acid contained in the extract. The seed of S. humilis Zucc. have been extracted by soxhletation with n-hexane as the solvent. The extract has been tested through in vitro antimicrobial bioassay toward Staphylococcus aureus and Escherichia coli by paper disc diffusion method. The fatty acid composition has been identified by fatty acid methyl esters method through gas chromatography coupled with mass spectroscopy. The result shows that the extract is more effective to inhibit the S. aureus activity with diameter range from 10 to 17 mm while E. coli tends to be resistance. The extract contains palmitic acid 15.49%; palmitoleic acid 0.28%; octadecanoic acid 14.04%; oleic acid 37.02 %; linoleic acid 24.70%; linolenic acid 7.33%; arachidic acid 0.84%; and tricosanoic acid 0.33%. The research confirms that S. humilis Zucc. seed oil contains polyunsaturated fatty acid and can be used as an antimicrobial agent.

IOP Conf. Ser.: Earth Environ. Sci. 251 012016

DOI: doi:10.1088/1755-1315/251/1/012016

No. Arsip : LIPI-20190427
Download Disini