Biodegradasi Aspal secara Simultan oleh Kombinasi Jamur Pestalotiopsis sp. dan Trametes hirsuta

Penulis: Dede Heri Yuli Yanto, Sanro Tachibana
The use of asphalt in road has contributed to contamination particularly by diffusing particles to water. Asphalt consists mostly of asphaltene instead of aliphatics, aromatics, and resins. Constrains in the biodegradation of asphalt have been attributed to the different susceptibilities of fractions to biodegradation by microorganism. During biodegradation, the composition of fractions may change to a higher ratio of asphaltene to aliphatic fraction, leading to lower biodegradability. Microorganisms that gow and produce highly degradative enzymes, resulting in a lower ratio of the asphaltene fraction, play a key role in accelerating biodegradation. However, identifying microorganisms with these characteristics is challenging because the compatibility model of PHCs, which place aliphatic and aromatic fractions at the outer layer and resin and asphaltene fractions at the inner layer, makes it difficult for a single microorganism to simultaneously attack all fractions. Fungal coculturing is a potential strategy that may accelerate biodegradation by enhancing the activities of enzymes responsible for degradation due to their synergistic effects. In the present study, a co-culture of Pestalotiopsis sp. NG007, an ascomycete fungus, with Trametes hirsuta D7, a basidiomycete fungus, simultaneously degraded asphalt in soil by using a synergistic mechanisms indicating may a high potential as an asphalt bioremediation agent in environmental contaminated with asphalt.

Prosiding Seminar Nasional XVIII MAPEKI 4-5 November 2015, Bandung

ISSN / ISBN / IBSN : ISSN: 2407-2036

No. Arsip : LIPI-16011