Effect of Pressure on the Supercritical CO2 Extraction of Asiaticoside from Centella asiatica

Penulis: Dewi Sondari, Tun Tedja Irawadi, Dwi Setyaningsih, and Silvester Tursiloadi
Asiaticoside as a bioactive component was extracted from Centella asiatica (L) Urb using supercritical (SC) CO2 fluid at operating pressure, temperature, extraction time and SC-CO2 flow rate in the range 10-27 MPa, 50 oC, 4 hours and 5.5 ml/min, respectively. In this experiment, the influence of pressure on the yield crude extract, the content asiaticoside as bioactive components, total phenolic and total triterpenoids contents from Centella asiatica extraction using supercritical CO2 will be seen, maceration extraction was used as the conventional technique for comparison purposes. Extraction yields of crude extract was measured using high performance liquid chromathography (HPLC). Result showed that the crude extract from Centella asiatica (L) Urb exhibits higher extraction yield with 9.23 mg/g of dry weight and 370.81g/mL of asiaticoside concentration at condition process 27 MPa. Highest total phenolic content was found at 74.28 mg/g gallic acid equivalents of Centella asiatica (L) Urb was extracted at maceration extraction, and triterpenoid content was found to be lowest in Centella asiatica (L) Urb (0.20 mg/g extract). In this study, the effect of pressure on the extraction of Centella asiatica using supercritical CO2 can significantly affect the asiaticoside concentration compared to maceration extraction, but does not affect to total phenolic and triterpenoids contents significantly. Keywords: HPLC, asiaticosida, phenolic, triterpenoid, supercritical CO2 fluids.

ADVANCED SCIENCE LETTERS, Volume 23, Number 12 (December 2017) pp.11635-12838 Index Scopus

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ISSN / ISBN / IBSN : ISSN: 1936-6612 (Print): EISSN: 1936-7317 (Online)

No. Arsip : LIPI-17180