Identification of Metabolite Products from Biotransformation of PAHs Mixture by a pH-saline Tolerant Fungus, Pestalotiopsis sp. NG007

Penulis: Dede Heri Yuli Yanto, Sanro Tachibana
In this study, the ability of Pestalotiopsissp. NG007 to degrade and transform PAHs was examined using four PAHs: acenaphthene (ACE), anthracene (ANT), chrysene (CHRY), and benzo[a]pyrene (BaP) in liquid medium. The experiments were conducted at different pH (312) and salinities (010 persen). The reHirsutasults showed that the biodegradation of PAHs mixture was dependent to pH and salinities. All the PAHs showed maximum degradation at range pH 3-5 and salinities 23.5 persen. The maximum degradation for ACE, ANT, CHRY, and BaP were 100 persen, 91 persen, 76 persen, and 68 persen, respectively. The biomass weight of strain NG007 increased from pH 311 (0.280.42 g/20 mL media), but dramatically decreased at pH 12 (0.20 g/20 mL media). In saline, strain NG007 grew higher than non-saline with optimum biomass was obtained at salinity of 2.5 persen. Analysis of metabolites using GC-MS showed that at least 11 compounds were detected. Strain NG007 degraded ACE via the formation of 1-acenaphthenol (I), acenaphthenon (II) and naphthalic anhydride (III). Anthraquinone (IV) detected in the culture can be identified as a metabolite from ANT degradation. In addition, the presence of 1-hydroxy-2-naphthoic acid (V) may indicate the metabolism of CHRY and BaP. Other compounds such as gentisic acid (VI), mandelic acid (VII), phthalic acid (VIII), cathecol (IX), benzaldehyde (X), and benzoic acid (XI) indicated that the biodegradation of the major metabolites into small compounds was occurred. This study demonstrated that strain NG007 may have the potential for degradation of PAHs in contaminated environments.

Proceedings of The 7th International Symposium of IWoRS 5-6 November 2015, Bandung

ISSN / ISBN / IBSN : ISSN: 2459-9867

No. Arsip : LIPI-16012