Rutaceae: conservation at Eka Karya Bali Botanic Garden and its in vitro antifungal activity screening

Penulis: Frelyta Ainuz Zahro

Several species of Rutaceae have been widely used and commercialized in all regions in Indonesia. Some species of Rutaceae are consumed as fresh fruit and traditional medicine for various kinds of diseases, as well as to add aroma to various Indonesian culinary. Since 1959, Eka Karya Bali Botanic Garden (Eka Karya BBG) has successfully collected dozens of Rutaceae species with unknown potential. In addition to reporting the conservation of Rutaceae in Eka Karya BBG, this study aimed to screen the anti- fungal activity of Rutaceae methanolic extract toward Aspergillus niger, Cladosporium sp., and Fusarium solani. Leaves of 13 species of Rutaceae (Boenninghausenia sp., Citrus aurantifolia, C. maxima, C. medica, Clausena sp., Melicope sp., Micromelum sp., Murraya paniculata, Toddalia sp., Zanthoxylum sp., Z. alatum, Z. limonella, and Z. ovalifolium) were collected, cleaned, air-dried, soaked in methanol for three days, then evaporated using a rotary evaporator to obtain the plant crude extract. The in vitro inhibitory assay was conducted by the diffusion method. As a result, only C. medica, Clausena sp., and Z. limonella exhibited antifungal activity against those tested fungi. Their antifungal activity increased on day 2 post-treatment but slowly decreased on day 3. Thus, the result of this experiment can be used as preliminary data to research Rutaceae plant extracts as an alternative method to control pathogenic fungi. However, further research is needed to maintain and increase its inhibitory effect

Wibawa, I.P.A.H., Li’aini, A.S., Andila, P.S., and Zahro’, F.A. (2021). Rutaceae: conservation at Eka Karya Bali Botanic Garden and its in vitro antifungal activity screening. Ilmu Pertanian (Agricultural Science), 6(2), pp. 108–116.

UGM

ISSN / ISBN / IBSN : ISSN 0126-4214 (print) ISSN 2527-7162 (onlin

DOI: DOI: doi.org/10.22146/ipas.60369

No. Arsip : LIPI-20220203
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